If more than one topical ophthalmic drug is being used, the drugs should be administered at least five minutes apart.
The effect of latanoprost is additive in combination with beta-adrenergic antagonists (timolol), adrenergic agonists (dipivalyl epinephrine), oral carbonic anhydrase inhibitors (acetazolamide) and at least partly additive with cholinergic agonists (pilocarpine).
The use of two or more prostaglandins, prostaglandin analogues or prostaglandin derivatives is not recommended.